Affiliation:
1. Division of Sexually Transmitted Diseases Laboratory Research, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
Abstract
The promoter region of the major iron-regulated protein of Neisseria gonorrhoeae, Fbp, has two regions that exhibit homology with the Escherichia coli consensus Fur-binding sequences. Gel retardation assays suggested that purified E. coli Fur bound to two sites within the Fbp promoter. The presence of a gonococcal Fur homolog was suggested by Southern hybridization under conditions of low stringency, which revealed a DNA locus that exhibited homology to the E. coli fur gene. Oligonucleotides derived from the conserved regions of fur genes of extremely diverse bacteria were used to amplify a 140-bp fragment of a putative gonococcal fur gene. This fragment was used to identify clones containing the entire gonococcal fur gene. After sequencing the gonococcal fur gene and its promoter region, we found that gonococcal Fur exhibited 50% identity with E. coli Fur at the amino acid level; however, it complemented two E. coli Fur- mutants. The presence of a Fur homolog in N. gonorrhoeae suggests that Fur-regulated genes are widely distributed among extremely diverse bacteria.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference51 articles.
1. Ausubel F. M. R. Brent R. E. Kingston D. Moore J. G. Seidman J. A. Smith and K. Struhl (ed.). 1987. Current protocols in molecular biology vol. 2 p. 4.4.2. John Wiley & Sons New York.
2. Molecular mechanisms of regulation of siderophore-mediated iron assimilation;Bagg A.;Microbiol. Rev.,1987
3. Molecular cloning and characterization of the structural gene for the major iron-regulated protein expressed by Neisseria gonorrhoeae;Berish S. A.;J. Exp. Med.,1990
4. A rapid alkaline extraction procedure for screening recombinant plasmid DNA;Birnboim H. C.;Nucleic Acids Res.,1979
5. Role of iron in bacterial infection;Bullen J.;Curr. Top. Microbiol. Immunol.,1978
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