EFFECT OF DRUG-RESISTANCE FACTOR R ON THE F PROPERTIES OF ESCHERICHIA COLI

Abstract

Hirota, Yukinori (Osaka University, Osaka, Japan), Yukinobu Nishimura, Frits Ørskov, and Ida Ørskov . Effect of drug-resistance factor R on the F properties of Escherichia coli . J. Bacteriol. 87: 341–351. 1964.—Infection of Escherichia coli male cells (Hfr or F + ) with resistance factor R results in the co-ordinate inhibition of several distinct functions of F factor: mating capacity to transfer chromosome by conjugation, production of f + antigen, and formation of receptors for the male-specific bacteriophages, f1 and ribonucleic acid phage. The i − mutant (R 100−1 ) of R factor, which was isolated from wild-type R factor (R 100 ), shows no inhibition of these F properties. Male R + 100−1 cells were autoagglutinable but the f + antigen was still present. When R-infected female cells had acquired the ability to form recombinants with an F − strain, they also had become autoagglutinable. The question of the presence of f + antigen in these strains was not solved. The cause of the autoagglutinability is not known, but it is not the result of loss of O antigen (rough autoagglutinability). Sensitivity to a phage tau , which can form plaques on female cells only, is not affected by the presence or absence of R factor. No difference in the pattern of segregation of recombinants was observed between the cross of Hfr R − × F − and that of Hfr R + × F − . These results indicate that R factor controls a key mechanism in the synthesis of “F substances” formed on the cell surface by the F factor.