Cellular fatty acid compositions of an unidentified organism and a bacterium associated with cat scratch disease

Author:

Moss C W1,Holzer G1,Wallace P L1,Hollis D G1

Affiliation:

1. Analytical Chemistry Laboratory, Division of Bacterial Diseases, Centers for Disease Control, Atlanta, Georgia 30333.

Abstract

The cellular fatty acid composition of a gram-negative bacterium associated with cat scratch disease was determined by capillary gas chromatography-mass spectrometry (GC-MS). The fatty acid profile of this organism was distinct from those of other bacteria we have tested and was characterized by an unknown acid which was identified as 11-methyloctadec-12-enoic acid. The position of the branched methyl group in this acid was established by GC-MS of the reduced acid, and the location of the double bond was confirmed by GC-MS analysis of dimethyl disulfide derivatives. Another clinical isolate with no known relationship to cat scratch disease but with similar morphological and biochemical features had a similar fatty acid profile, including 11-methyloctadec-12-enoic acid.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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