Heterologous Expression of an Entamoeba histolytica Chitin Synthase in Saccharomyces cerevisiae

Author:

Van Dellen Katrina L.1,Bulik Dorota A.1,Specht Charles A.2,Robbins Phillips W.1,Samuelson John C.1

Affiliation:

1. Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine

2. Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118

Abstract

ABSTRACT Chitin in the cyst wall of Entamoeba histolytica is made by two chitin synthases (Chs), one of which is unique (EhCHS-1) and one of which resembles those of insects and nematodes (EhCHS-2). EhCHS-1 is deposited chitin in the lateral wall of transformed Saccharomyces cerevisiae Chs mutants, independent of accessory proteins (Chs4p to Chs7p) required by yeast Chs3p.

Publisher

American Society for Microbiology

Subject

Molecular Biology,General Medicine,Microbiology

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