The low-affinity component of Saccharomyces cerevisiae maltose transport is an artifact

Author:

Benito B1,Lagunas R1

Affiliation:

1. Instituto de Investigaciones Biomédicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain.

Abstract

It has been reported by several laboratories that maltose transport in Saccharomyces cerevisiae consists of two components with high- and low-affinity constants for maltose. We have investigated the characteristics of the low-affinity component and have found that it shows an abnormal behavior without similarity to any transport mechanism described in this organism. The results strongly indicate that this apparent transport activity is due not to a genuine transport process but to nonspecific binding of maltose to the cell wall and plasma membrane.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference23 articles.

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2. Identification of two forms of the maltose transport system in Saccharomyces cerevisiae and their regulation by catabolite inactivation;Busturia A.;Biochim. Biophys. Acta 820'.324-326.,1985

3. Catabolite inactivation of the glucose transport system in Saccharomyces cerevisiae;Bsturia A.;J. Gen. Microbiol.,1986

4. Cartwight C. P. A. H. Rose J. Calderbauk and M. H. J. Keenan. 1989. Solute transport p. 5-49. In A. H. Rose and J. S. Harrison (ed.) The yeast vol. 3 2nd ed. Academic Press New York.

5. MALII and AL4L61 encode the inducible high-affinity maltose transporters of Saccharomyces cerevisiae;Cheng Q.;J. Bacteriol.,1991

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