Author:
Maltz Michele A.,Weiss Brian L.,O'Neill Michelle,Wu Yineng,Aksoy Serap
Abstract
ABSTRACTMany bacteria successfully colonize animals by forming protective biofilms. Molecular processes that underlie the formation and function of biofilms in pathogenic bacteria are well characterized. In contrast, the relationship between biofilms and host colonization by symbiotic bacteria is less well understood. Tsetse flies (Glossinaspp.) house 3 maternally transmitted symbionts, one of which is a commensal (Sodalis glossinidius) found in several host tissues, including the gut. We determined thatSodalisforms biofilms in the tsetse gut and that this process is influenced by theSodalisouter membrane protein A (OmpA). MutantSodalisstrains that do not produce OmpA (SodalisΔOmpA mutants) fail to form biofilmsin vitroand are unable to colonize the tsetse gut unless endogenous symbiotic bacteria are present. Our data indicate that in the absence of biofilms,SodalisΔOmpA mutant cells are exposed to and eliminated by tsetse's innate immune system, suggesting that biofilms helpSodalisevade the host immune system. Tsetse is the sole vector of pathogenic African trypanosomes, which also reside in the fly gut. Acquiring a better understanding of the dynamics that promoteSodaliscolonization of the tsetse gut may enhance the development of novel disease control strategies.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference53 articles.
1. Genome size determination and coding capacity of Sodalis glossinidius, an enteric symbiont of tsetse flies, as revealed by hybridization to Escherichia coli gene arrays;Akman;J. Bacteriol.,2001
2. Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia;Akman;Nat. Genet.,2002
3. Mycetome endosymbionts of tsetse flies constitute a distinct lineage related to Enterobacteriaceae;Aksoy;Insect Mol. Biol.,1995
4. Analysis of milk gland structure and function in Glossina morsitans: milk protein production, symbiont populations and fecundity;Attardo;J. Insect Physiol.,2008
5. Hha, YbaJ, and OmpA regulate Escherichia coli K12 biofilm formation and conjugation plasmids abolish motility;Barrios;Biotechnol. Bioeng.,2006
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