Effect of Iron Concentration on the Growth Rate of Pseudomonas syringae and the Expression of Virulence Factors in hrp -Inducing Minimal Medium-Reference-Cited by-同舟云学术

Effect of Iron Concentration on the Growth Rate of Pseudomonas syringae and the Expression of Virulence Factors in hrp -Inducing Minimal Medium

Published:2009-05 Issue:9 Volume:75 Page:2720-2726
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Kim Beum Jun¹,Park Joon Ho¹²,Park Tai Hyun¹²,Bronstein Philip A.³,Schneider David J.³,Cartinhour Samuel W.³,Shuler Michael L.¹⁴

Affiliation:

1. School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York 14853

2. School of Chemical and Biological Engineering, Seoul National University, Gwanak-gu, Sillim-dong San 56-1, Seoul 151-744, South Korea

3. U.S. Department of Agriculture, Agricultural Research Service, and Department of Plant Pathology and Plant-Microbe Interactions, Cornell University, Ithaca, New York 14853

4. Department of Biomedical Engineering, Cornell University, Ithaca, New York 14853

Abstract

ABSTRACT Although chemically defined media have been developed and widely used to study the expression of virulence factors in the model plant pathogen Pseudomonas syringae , it has been difficult to link specific medium components to the induction response. Using a chemostat system, we found that iron is the limiting nutrient for growth in the standard hrp -inducing minimal medium and plays an important role in inducing several virulence-related genes in Pseudomonas syringae pv. tomato DC3000. With various concentrations of iron oxalate, growth was found to follow Monod-type kinetics for low to moderate iron concentrations. Observable toxicity due to iron began at 400 μM Fe 3+ . The kinetics of virulence factor gene induction can be expressed mathematically in terms of supplemented-iron concentration. We conclude that studies of induction of virulence-related genes in P. syringae should control iron levels carefully to reduce variations in the availability of this essential nutrient.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02738-08

Reference29 articles.

1. Büttner, D., and U. Bonas. 2003. Common infection strategies of plant and animal pathogenic bacteria. Curr. Opin. Plant Biol.6:312-319.

2. Byers, B. R., and J. E. L. Arceneaux. 1998. Microbial iron transport: iron acquisition by pathogenic microorganisms, p. 37-66. In A. Sigel and H. Sigel (ed.), Metal ions in biological systems: iron transport and storage in microorganisms, plants, and animals, vol. 35. Marcel Dekker, Inc., New York, NY.

3. Earhart, C. F. 1996. Uptake and metabolism of iron and molybdenum, p. 1075-1090. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., vol. 1. ASM Press, Washington, DC.

4. Goldberg, I., and Z. Er-el. 1981. The chemostat—an efficient technique for medium optimization. Process Biochem.16:2-8.

5. Gross, D. C. 1985. Regulation of syringomycin synthesis in Pseudomonas syringae pv. syringae and defined conditions for its production. J. Appl. Bacteriol.58:167-174.

Cited by 41 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Insights on the synthesis of iron-oxide nanoparticles and the detection of iron-reducing genes from soil microbes;Colloids and Surfaces C: Environmental Aspects;2024-11

2. In Silico Identification of Cadmium Binding Protein and its Secreted Metalloproteins in Stenotrophomonas maltophilia;Current Chemical Biology;2023-09

3. Roles of Fe(II), Fe(III) and Fe0 in denitrification and anammox process: Mechanisms, advances and perspectives;Journal of Water Process Engineering;2023-07

4. Production and characterization of lipopeptide biosurfactant from a new strain of Pseudomonas antarctica 28E using crude glycerol as a carbon source;RSC Advances;2023

5. Removal of N and P in a Rotating Biological Contactor Plant: Case Study Agnita, Romania;Water;2022-11-14