Role of NifS in maturation of glutamine phosphoribosylpyrophosphate amidotransferase-Reference-Cited by-同舟云学术

Role of NifS in maturation of glutamine phosphoribosylpyrophosphate amidotransferase

Published:1997-12 Issue:23 Volume:179 Page:7587-7590
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Chen S¹,Zheng L¹,Dean D R¹,Zalkin H¹

Affiliation:

1. Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907, USA.

Abstract

Glutamine phosphoribosylpyrophosphate amidotransferase from Bacillus subtilis is synthesized as an inactive precursor that requires two maturation steps: incorporation of a [4Fe-4S] center and cleavage of an 11-residue NH2-terminal propeptide. Overproduction from a multicopy plasmid in Escherichia coli leads to the formation of soluble proenzyme and mature enzyme forms as well as a small fraction of insoluble proenzyme. Heterologous expression of Azotobacter vinelandii nifS from a compatible plasmid increased the maturation of the soluble proenzyme three- to fourfold without influencing the content of the insoluble fraction. These results support a role for NifS in heterologous Fe-S cluster assembly and enzyme maturation.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.179.23.7587-7590.1997

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