Potential for Erroneous Results Indicating Resistance When Using the Bactec MGIT 960 System for Testing Susceptibility of Mycobacterium tuberculosis to Pyrazinamide

Author:

Chedore Pamela1,Bertucci Lina1,Wolfe Joyce2,Sharma Meenu2,Jamieson Frances1

Affiliation:

1. Ontario Agency for Health Protection and Promotion, Public Health Laboratory, Toronto, Ontario, Canada

2. National Reference Centre for Mycobacteriology, National Microbiology Laboratory, Canadian Science Centre for Human and Animal Health, Winnipeg, Manitoba, Canada

Abstract

ABSTRACT During susceptibility testing of 743 isolates of Mycobacterium tuberculosis to pyrazinamide (PZA) using the Bactec 960 system, 57 (7.7%) isolates showed PZA resistance. Repeat testing of resistant isolates with the Bactec 460 reference method confirmed 33 (4.4%) of these isolates as resistant, and 24 (3.2%) were susceptible. Erroneous results for resistance with the Bactec 960 were confirmed by testing the 24 discordant isolates for pyrazinamidase and mutations in the pncA gene.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference15 articles.

1. Aono, A., K. Hirano, S. Hamasaki, and C. Abe. 2002. Evaluation of BACTEC MGIT 960 PZA medium for susceptibility testing of Mycobacterium tuberculosis to pyrazinamide (PZA): compared with the results of pyrazinamidase assay and Kypkuto PZA test. Diagn. Microbiol. Infect. Dis.44:347-352.

2. Bactec MGIT 960 system user's manual. 1999

3. Clark, R. B., M. A. Lewinski, M. J. Loeffelholz, and R. J. Tibbetts. 2009. Cumitech 31A, Verification and validation of procedures in the clinical microbiology laboratory, p 14. Coordinating ed., S. E. Sharp. ASM Press, Washington, DC.

4. Susceptibility testing of Mycobacteria Nocardiae and other aerobic Actinomycetes 2003 vol. 23

5. Comparison of Phenotypic and Genotypic Methods for Pyrazinamide Susceptibility Testing with Mycobacterium tuberculosis

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