Two-Step Scheme for Rapid Identification and Differentiation of Legionella pneumophila and Non- Legionella pneumophila Species-Reference-Cited by-同舟云学术

Two-Step Scheme for Rapid Identification and Differentiation of Legionella pneumophila and Non- Legionella pneumophila Species

Published:2010-02 Issue:2 Volume:48 Page:433-439
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Zhan Xiao-Yong¹,Li Lian-Qing²,Hu Chao-Hui¹,Zhu Qing-Yi¹

Affiliation:

1. Guangzhou Kingmed Center for Clinical Laboratory, Guangzhou 510330, Guangdong, China

2. Shanxi Center for Clinical Laboratories, Taiyuan 030012, Shanxi, China

Abstract

ABSTRACT A rapid two-step scheme based on PCR amplification and enzymatic digestion analysis of a 226-bp fragment of the 16S rRNA gene was developed to identify the Legionella genus by PCR amplification and to differentiate the Legionella pneumophila and non- Legionella pneumophila species by enzymatic digestion analysis. Among 42 ATCC strains (16 strains of L. pneumophila and 26 strains of non- L. pneumophila ) and 200 Legionella isolates from environmental water samples, including pools, rivers, lakes, and cooling towers in Guangdong province, 99.59% of L. pneumophila and non- L. pneumophila strains were correctly identified and differentiated by this scheme. The procedure of this two-step identification and differentiation scheme is simple and takes only about 4 h. These results suggest that this two-step scheme provides a simple and convenient method for the rapid identification and differentiation of L. pneumophila and non- L. pneumophila species.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01778-09

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