Affiliation:
1. Department of Biological Sciences, Mississippi State University, Mississippi State, Mississippi
2. College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi
Abstract
ABSTRACT
Listeria monocytogenes
is a gram-positive, food-borne pathogen that causes disease in both humans and animals. There are three major genetic lineages of
L. monocytogenes
and 13 serovars. To further our understanding of the differences that exist between different genetic lineages/serovars of
L. monocytogenes
, we analyzed the global protein expression of the serotype 1/2a strain EGD and the serotype 4b strain F2365 during early-stationary-phase growth at 37°C. Using multidimensional protein identification technology with electrospray ionization tandem mass spectrometry, we identified 1,754 proteins from EGD and 1,427 proteins from F2365, of which 1,077 were common to both. Analysis of proteins that had significantly altered expression between strains revealed potential biological differences between these two
L. monocytogenes
strains. In particular, the strains differed in expression of proteins involved in cell wall physiology and flagellar biosynthesis, as well as DNA repair proteins and stress response proteins.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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