Affiliation:
1. Department of Biochemistry, New York University School of Medicine, New York, New York 10016
Abstract
Circular deoxyribonucleic acid was isolated from
Shigella dysenteriae
Y6R and was found to consist of six species having molecular weights of 10
6
, 1.3 × 10
6
, 2.6 × 10
6
, 3.8 × 10
6
, 20 × 10
6
, and 24 × 10
6
daltons. These size classes were partially resolved by sucrose density gradient centrifugation. The minicircles (10
6
and 1.3 × 10
6
) were found to have a buoyant density in CsCl of 1.710 g/ml. The 3.8 × 10
6
dalton class had a density of 1.707 g/ml. The two largest species had a density of 1.702 g/ml. Two other strains,
S. sonnei
II and
S. dysenteriae
60, also contained circular deoxyribonucleic acid.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
18 articles.
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