Affiliation:
1. Cancer Research Institute and Department of Medicine, University of California School of Medicine, San Francisco, California 94122
Abstract
A technique is described for the measurement of listericidal activity of human macrophages grown from blood monocytes. Phagocytosis of
Listeria monocytogenes
was inhibited by a glycolytic poison (NaF) but was unaffected by anaerobic conditions, cyanide, or 2,4-dinitrophenol (DNP). Killing by macrophages was slower than that by neutrophils, and
Listeria
phagocytized by macrophages began to synthesize deoxyribonucleic acid within 3 hr of the time of ingestion. Differentiated macrophages ingested and killed more organisms per cell than newly isolated monocytes. Maximal killing of
Listeria
required oxygen but was unaffected by cyanide or DNP. Macrophages isolated from patients with chronic intracellular infection (leprosy, tuberculosis, fungal diseases) and from patients with active Hodgkin's disease were more bactericidal than macrophages from normal subjects.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
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