A PCR-Based Method for Distinguishing between Two Common Beehive Bacteria, Paenibacillus larvae and Brevibacillus laterosporus

Author:

Berg Jordan A.1ORCID,Merrill Bryan D.1ORCID,Breakwell Donald P.1,Hope Sandra1ORCID,Grose Julianne H.1ORCID

Affiliation:

1. Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah, USA

Abstract

16S rRNA gene sequencing in bacteria has long been held as the gold standard for typing bacteria and, for the most part, is an excellent method of taxonomically identifying different bacterial species. However, the high level of 16S rRNA sequence similarity of some published strains of P. larvae and B. laterosporus , as well as possible horizontal gene transfer events within their shared ecological niche, complicates the use of 16S rRNA sequence as an effective molecular marker for differentiating these two species. Additionally, shared characteristics of these bacteria limit the effectiveness of using traditional phenotypic identification assays, such as the catalase test. The results from this study provide PCR methods to quickly differentiate between these two genera and will be useful when studying Brevibacillus , Paenibacillus , and other disease-relevant bacteria commonly found in beehives.

Funder

Brigham Young University

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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