Visual Reading of Enzyme Immunofluorescence Assays for Human Cytomegalovirus Antibodies

Author:

Forghani Bagher1,Dennis Juanita1,Schmidt Nathalie J.1

Affiliation:

1. Viral and Rickettsial Disease Laboratory, California Department of Health Services, Berkeley, California 94704

Abstract

Enzyme immunofluorescence assays for cytomegalovirus antibodies could be read satisfactorily using a light box with ultraviolet illumination. Higher antibody titers were obtained with a fluorogenic substrate than with a color-producing substrate.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference10 articles.

1. A multi-purpose laboratory light box;Dennis J.;Am. J. Med. Technol.,1975

2. Enzyme-linked immunosorbent assay;Engvall E.;ELISA. J. Immunol.,1972

3. Kinetic behaviour of calf-intestinal alkaline phosphatase with 4-methylumbelliferyl phosphate;Fernley H. N.;Biochem. J.,1965

4. Antigen requirements, sensitivity, and specificity of enzyme immunoassays for measles and rubella viral antibodies;Forghani B.;J. Clin. Microbiol.,1979

5. Antibody assays for varicella:zoster: comparison of enzyme immunoassay with neutralization, immune adherence hemagglutination, and complement fixation;Forghani B.;J. Clin. Microbiol.,1978

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