Evaluation of the Prototype Roche DNA Amplification Kit Incorporating the New SSK145 and SKCC1B Primers in Detection of Human Immunodeficiency Virus Type 1 DNA in Zimbabwe-Reference-Cited by-同舟云学术

Evaluation of the Prototype Roche DNA Amplification Kit Incorporating the New SSK145 and SKCC1B Primers in Detection of Human Immunodeficiency Virus Type 1 DNA in Zimbabwe

Published:1999-11 Issue:11 Volume:37 Page:3569-3571
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Zijenah Lynn S.¹,Humphrey Jean²,Nathoo Kussum²,Malaba Lucie³,Zvandasara Parteson⁴,Mahomva Agnes⁵,Iliff Peter²,Mbizvo Michael T.⁴

Affiliation:

1. Departments of Immunology,1

2. Paediatrics,2

3. Nutrition,3 and

4. Obstetrics and Gynaecology,4 University of Zimbabwe, and

5. Harare City Health Department,5 Harare, Zimbabwe

Abstract

ABSTRACT We assessed the sensitivity and specificity of a newly developed DNA PCR kit (Roche Diagnostic Corporation, Indianapolis, Ind.) that incorporates primers for all the group M viruses for the detection of human immunodeficiency virus (HIV) type 1 (HIV-1) infection in Zimbabwe. A total of 202 whole-blood samples from adults whose HIV status was known were studied. This included 100 HIV-1-positive and 102 HIV-1-negative samples selected on the basis of concordant results obtained with two enzyme-linked immunosorbent assay kits. The prototype Roche DNA PCR assay had a 100% sensitivity for the detection of HIV-1 DNA and a specificity of 100%. We conclude that the new Roche DNA PCR kit is accurate for the detection of HIV DNA in Zimbabwean samples, in which HIV-1 subtype C dominates.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.37.11.3569-3571.1999

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