Affiliation:
1. Department of Veterinary Microbiology and Pathology1 and
2. Washington Animal Disease Diagnostic Laboratory,2 Washington State University, Pullman, Washington
Abstract
ABSTRACT
The routine diagnosis of
Neospora caninum
abortion is based upon histopathologic changes in fetal tissues and identification of tissue parasites by immunohistochemistry. Confirmation of
N. caninum
infection by immunohistochemistry has low sensitivity. In the present study, we examined the utility of PCR in detecting
N. caninum
infection in fetal tissues from spontaneous bovine abortion. DNA was obtained from fresh and formalin-fixed tissues from 61 bovine fetuses submitted for abortion diagnosis. Histopathology and immunohistochemistry determined the true status of
N. caninum
infection in each fetus. In formalin-fixed paraffin-embedded tissues, PCR detected
N. caninum
DNA in 13 of 13 true-positive fetuses (100%) and in 1 of 16 true-negative fetuses (6%). In fresh or frozen tissues, PCR detected
N. caninum
DNA in 10 of 13 true-positive fetuses (77%) and 0 of 11 true-negative fetuses (0%). PCR also detected
N. caninum
DNA in 6 of 8 fetuses that had typical lesions of
N. caninum
but were immunohistochemistry negative, indicating a higher sensitivity of PCR in comparison to that of immunohistochemistry.
N. caninum
DNA was amplified most consistently from brain tissue. PCR detection of
N. caninum
DNA in formalin-fixed, paraffin-embedded tissues was superior to that in fresh tissues, presumably because of the increased accuracy of sample selection inherent in histologic specimens.
Publisher
American Society for Microbiology
Reference41 articles.
1. Protozoal causes of reproductive failure in domestic ruminants;Anderson M. L.;Vet. Clin. N. Am. Food Anim. Pract.,1994
2. Neospora-like protozoan infection as a major cause of abortion in California dairy cattle;Anderson M. L.;J. Am. Vet. Med. Assoc.,1991
3. Evaluation of abortions in cattle attributable to neosporosis in selected dairy herds in California;Anderson M. L.;J. Am. Vet. Med. Assoc.,1995
4. Bovine fetal encephalitis and myocarditis associated with protozoal infections;Barr B. C.;Vet. Pathol.,1990
5. Neospora-like protozoal infections associated with bovine abortions;Barr B. C.;Vet. Pathol.,1991
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