Rapid Identification of Campylobacter , Arcobacter , and Helicobacter Isolates by PCR-Restriction Fragment Length Polymorphism Analysis of the 16S rRNA Gene-Reference-Cited by-同舟云学术

Rapid Identification of Campylobacter , Arcobacter , and Helicobacter Isolates by PCR-Restriction Fragment Length Polymorphism Analysis of the 16S rRNA Gene

Published:1999-12 Issue:12 Volume:37 Page:4158-4160
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Marshall Stephen M.¹,Melito Pasquale L.²,Woodward David L.²,Johnson Wendy M.²,Rodgers Frank G.²,Mulvey Michael R.²

Affiliation:

1. National Research Council Canada, Institute for Biological Sciences, Ottawa, Ontario K1A 0R6,1 Canada

2. Canadian Science Centre for Human and Animal Health, Bureau of Microbiology/Laboratory Center for Disease Control, Winnipeg, Manitoba R3E 3R2,2 and

Abstract

ABSTRACT A rapid two-step identification scheme based on PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the 16S rRNA gene was developed in order to differentiate isolates belonging to the Campylobacter , Arcobacter , and Helicobacter genera. For 158 isolates (26 reference cultures and 132 clinical isolates), specific RFLP patterns were obtained and species were successfully identified by this assay.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.37.12.4158-4160.1999

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