Novel Method for Rapid Determination of Clarithromycin Sensitivity in Helicobacter pylori-Reference-Cited by-同舟云学术

Novel Method for Rapid Determination of Clarithromycin Sensitivity in Helicobacter pylori

Published:1999-11 Issue:11 Volume:37 Page:3746-3748
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Gibson J. R.¹,Saunders N. A.²,Burke B.¹,Owen R. J.¹

Affiliation:

1. Helicobacter Reference Unit, Laboratory of Enteric Pathogens,1 and

2. Molecular Biology Unit, Virus Reference Division,2 Central Public Health Laboratory, London NW9 5HT, United Kingdom

Abstract

ABSTRACT A novel PCR-hybridization assay, performed in single closed capillaries, was developed to detect clarithromycin resistance-associated gene mutations in Helicobacter pylori . Mutations were detected by thermal analysis in 33 of 34 (97%) resistant isolates but not in 66 isolates determined to be sensitive by conventional antibiotic assays. The method was rapid and reproducible, and it reduced PCR product contamination risk.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.37.11.3746-3748.1999

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