Affiliation:
1. Departments of Laboratory Medicine1 and
2. Internal Medicine,2 National Taiwan University Hospital, and
3. School of Medical Technology, National Taiwan University College of Medicine,3 Taipei, Taiwan
Abstract
ABSTRACT
Sixty-seven clinical isolates of
Streptococcus pneumoniae
(40 of serotype 23F, 19 of serotype 19F, and 8 of serotype 6B) with decreased susceptibilities to penicillin and erythromycin were characterized by antimicrobial susceptibility patterns; DNA restriction endonuclease cleavage profiles of the penicillin-binding protein genes
pbp1a
,
pbp2b
, and
pbp2x
; random amplified polymorphic DNA (RAPD) patterns generated by arbitrarily primed PCR; and chromosomal macrorestriction profiles based on pulsed-field gel electrophoresis. A total of 22 clones (identical or closely related pulsotypes and identical RAPD patterns) were identified; 14 clones of 23F, 6 of 19F, and 2 of 6B. Three 23F clones (26 isolates) and one 19F clone (9 isolates) expressed high-level resistance to penicillin, cefotaxime, and erythromycin (MICs ≥ 256 μg/ml). These data strongly suggest that multiple high-level penicillin-, extended-spectrum cephalosporin-, and macrolide-resistant clones of
S. pneumoniae
have been disseminated in Taiwan.
Publisher
American Society for Microbiology
Cited by
31 articles.
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