Phospholipids of Thiobacillus thiooxidans

Author:

Shively J. M.1,Benson A. A.2

Affiliation:

1. Department of Microbiology, University of Nebraska, Lincoln, Nebraska 68508

2. Department of Marine Biology, Scripps Institution of Oceanography, University of California at San Diego, La Jolla, California 92037

Abstract

Cells and spent growth media from sulfur- and thiosulfate-grown cultures of Thiobacillus thiooxidans were analyzed. The phosphatides were examined by thinlayer chromatography, and the products of their hydrolysis by hydrochloric acid and methanolic potassium hydroxide were separated by paper chromatography. The phospholipids in both cells and spent growth media were identified as phosphatidyl ethanolamine, phosphatidyl- N -monomethylethanolamine, phosphatidyl glycerol, and diphosphatidyl glycerol. These comprised about 97% of the total lipid phosphorus. Lyso-phosphatidyl- N -monomethylethanolamine and lysophosphatidyl glycerol accounted for the remaining 3%. The percentage of the total lipid phosphorus accounted for by each phospholipid depended on the age of the culture.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference27 articles.

1. AMERICAN PUBLIC HEALTH ASSOCIATION. 1965. Standard methods for the examination of water and waste-water 12 ed. American Public Health Association Inc. New York.

2. Plant phospholipids. I. Identification of the phosphatidyl glycerols;BENSON A. A.;Biochim. Biophys. Acta,1958

3. Plant phospholipids. III. Identification of diphosphatidyl glycerol;BENSON A. A.;Biochim. Biophys. Acta,1960

4. A rapid method of total lipid extraction and purification;BLIGH E. G.;Can. J. Biochem. Physiol.,1959

5. Monoand dimethylethanolamine isolated from ratliver phospholipids;BREMER J.;Biochim. Biophys. Acta,1959

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