Lab-on-Chip-Based Platform for Fast Molecular Diagnosis of Multidrug-Resistant Tuberculosis-Reference-Cited by-同舟云学术

Lab-on-Chip-Based Platform for Fast Molecular Diagnosis of Multidrug-Resistant Tuberculosis

Published:2015-12 Issue:12 Volume:53 Page:3876-3880
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Cabibbe Andrea M.¹,Miotto Paolo¹,Moure Raquel²,Alcaide Fernando²,Feuerriegel Silke³,Pozzi Gianni⁴,Nikolayevskyy Vladislav⁵⁶,Drobniewski Francis⁵⁶,Niemann Stefan³,Reither Klaus⁷⁸,Cirillo Daniela M.¹

Affiliation:

1. Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy

2. Servei de Microbiologia, Hospital Universitari de Bellvitge-IDIBELL, Universitat de Barcelona, Barcelona, Spain

3. Molecular Mycobacteriology, Research Center Borstel, Borstel, Germany

4. Laboratory of Molecular Microbiology and Biotechnology, Department of Medical Biotechnologies, University of Siena, Siena, Italy

5. Clinical TB and HIV Group, Blizard Institute, Queen Mary University of London, London, United Kingdom

6. Department of Infectious Diseases and Immunity, Imperial College London, London, United Kingdom

7. Swiss Tropical and Public Health Institute, Basel, Switzerland

8. Ifakara Health Institute, Bagamoyo, United Republic of Tanzania

Abstract

ABSTRACT We evaluated the performance of the molecular lab-on-chip-based VerePLEX Biosystem for detection of multidrug-resistant tuberculosis (MDR-TB), obtaining a diagnostic accuracy of more than 97.8% compared to sequencing and MTBDR plus assay for Mycobacterium tuberculosis complex and rifampin and isoniazid resistance detection on clinical isolates and smear-positive specimens. The speed, user-friendly interface, and versatility make it suitable for routine laboratory use.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01824-15

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