Enhanced isolation of respiratory syncytial virus in cell culture-Reference-Cited by-同舟云学术

Enhanced isolation of respiratory syncytial virus in cell culture

Published:1986-04 Issue:4 Volume:23 Page:800-802
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Arens M Q,Swierkosz E M,Schmidt R R,Armstrong T,Rivetna K A

Abstract

During two winter seasons, we found that the combination of WI-38 or MRC-5 human lung fibroblasts plus primary rhesus monkey kidney (RhMK) and HEp-2 cell cultures yielded maximal isolation of respiratory syncytial virus. Cytopathic effects (CPE) developed earliest in RhMK cells and slowest in the human fibroblast lines. In RhMK cells, 50% of ultimately positive cultures showed CPE in 5 days, and 90% of positive cultures showed CPE within 7 days during both respiratory syncytial virus seasons.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.23.4.800-802.1986

Reference8 articles.

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