Papilloma formation by cottontail rabbit papillomavirus requires E1 and E2 regulatory genes in addition to E6 and E7 transforming genes

Author:

Wu X1,Xiao W1,Brandsma J L1

Affiliation:

1. Section of Comparative Medicine, Yale University School of Medicine, New Haven, Connecticut 06510.

Abstract

The present study used the cottontail rabbit papillomavirus DNA-rabbit system to evaluate whether the regulatory genes E1 and E2 and the transforming gene E6 are required for papilloma formation. Frameshift mutations were generated in the individual genes in the context of a full-length cottontail rabbit papillomavirus genome, and the mutant DNAs were intradermally inoculated into domestic rabbits. None of the mutants induced papillomas. Marker rescue experiments confirmed that the defects were due to mutations that we deliberately introduced. Marker rescue also confirmed our previous report that the upstream region of E7 around position 9 was critical for papilloma induction. These results demonstrate that the E1 and E2 regulatory genes as well as the E6 and E7 transforming genes are each required for papilloma formation. Each gene may provide molecular targets for therapeutic intervention.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Reference63 articles.

1. Neuroepithelial carcinomas in mice transgenic with human papillomavirus type 16 E6/E7 ORFs;Arbeit J. M.;Am. J. Pathol.,1993

2. Baker C. C. 1987. Sequence analysis of papillomavirus genomes p. 321-385. In N. Salzman and P. M. Howley (ed.) The Papovaviridae vol. 2. Plenum Publishing Corp. New York.

3. Transcription of the cottontail rabbit papillomavirus early region and identification of two E6 polypeptides in COS-7 cells;Barbosa M. S.;J. Virol.,1987

4. Brandsma J. L. Unpublished data.

5. Infectious virus replication in papillomas induced by molecularly cloned cottontail rabbit papillomavirus DNA;Brandsma J. L.;J. Virol.,1993

Cited by 46 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Animal Models for Infectious Disease Vaccine Development;A Comprehensive Guide to Toxicology in Nonclinical Drug Development;2024

2. Modeling HPV-Associated Disease and Cancer Using the Cottontail Rabbit Papillomavirus;Viruses;2022-09-04

3. Human papillomavirus type 16 E6 induces cell competition;PLOS Pathogens;2022-03-23

4. Human Papillomavirus Type 16 E6 induces cell competition;2021-07-06

5. The rabbit papillomavirus model: a valuable tool to study viral–host interactions;Philosophical Transactions of the Royal Society B: Biological Sciences;2019-04-08

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3