Enhanced Expression of Endochitinase in Trichoderma harzianum with the cbh1 Promoter of Trichoderma reesei

Author:

Margolles-Clark E,Harman G E,Penttila M

Abstract

Production of extracellular endochitinase could be increased 5-fold in the mycoparasite fungus Trichoderma harzianum by using the cellulase promoter cbh1 of Trichoderma reesei, whereas the total endochitinase activity increased 10-fold. The cbh1 promoter was not expressed on glucose and sucrose in T. harzianum and was induced by sophorose and on cellulase-inducing medium. The endogenous endochitinase gene was expressed at a low basal level on glucose and sucrose. No specific induction by crab shell chitin or sophorose was observed.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference18 articles.

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3. Antifungal alkyl pyrones of Trichoderma harzianum;Claydon N.;Trans. Br. Mycol. Soc.,1987

4. Cloning and characterization of a chitinase (CHIT42) cDNA from the mycoparasitic fungus Trichoderma harzianum;García I.;Curr. Genet.,1994

5. Isolation and sequence of an endochitinase-encoding gene from a cDNA library of Trichoderma harzianum;Hayes C. K.;Gene,1994

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