Affiliation:
1. Department of Biology, University of Konstanz, D-78457 Konstanz, Germany
Abstract
ABSTRACT
Bacillus subtilis
can utilize maltose and maltodextrins that are derived from polysaccharides, like starch or glycogen. In this work, we show that maltose is taken up by a member of the phosphoenolpyruvate-dependent phosphotransferase system and maltodextrins are taken up by a maltodextrin-specific ABC transporter. Uptake of maltose by the phosphoenolpyruvate-dependent phosphotransferase system is mediated by maltose-specific enzyme IICB (MalP; synonym, GlvC), with an apparent
K
m
of 5 μM and a
V
max
of 91 nmol · min
−1
· (10
10
CFU)
−1
. The maltodextrin-specific ABC transporter is composed of the maltodextrin binding protein MdxE (formerly YvdG), with affinities in the low micromolar range for maltodextrins, and the membrane-spanning components MdxF and MdxG (formerly YvdH and YvdI, respectively), as well as the energizing ATPase MsmX. Maltotriose transport occurs with an apparent
K
m
of 1.4 μM and a
V
max
of 4.7 nmol · min
−1
· (10
10
CFU)
−1
.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference47 articles.
1. Alting-Mees, M. A., and J. M. Short. 1989. pBluescript II: gene mapping vectors. Nucleic Acids Res. 17 : 9494.
2. Boos, W., and J. M. Lucht. 1996. Periplasmic binding protein-dependent ABC transporters, p. 1175-1209. In C. C. Lin, R. B. Loe, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed. ASM Press, Washington, D.C.
3. Maltose/Maltodextrin System of
Escherichia coli
: Transport, Metabolism, and Regulation
4. Brent, R., and M. Ptashne. 1981. Mechanism of action of the lexA gene product. Proc. Natl. Acad. Sci. USA 78 : 4202-4208.
5. Cho, H. Y., Y. W. Kim, T. J. Kim, H. S. Lee, D. Y. Kim, J. W. Kim, Y. W. Lee, S. Leed, and K. H. Park. 2000. Molecular characterization of a dimeric intracellular maltogenic amylase of Bacillus subtilis SUH4-2. Biochim. Biophys. Acta 1478 : 333-340.
Cited by
75 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献