Affiliation:
1. National Communicable Disease Center, Atlanta, Georgia 30333
Abstract
To achieve a rapid identification of the cell wall components of actinomycetes, several modifications of the procedure of Cummins and Harris were developed. Purified cell walls were prepared by extraction of whole cells with 0.1 N NaOH. A 2-hr 2 N HCl hydrolysate was prepared for identification of sugars. A 2-hr 6 N HCl hydrolysate was prepared for amino acid analysis. Two-dimensional paper chromatography was run on 19-cm papers. Total time required for a single analysis was approximately 24 hr. This procedure gave qualitative results which were completely satisfactory for differentiation of certain species in the genus
Actinomyces
and in related genera.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Cited by
10 articles.
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