Comparison of Madin-Darby canine kidney cells (MDCK) with a green monkey continuous cell line (Vero) and human lung embryonated cells (MRC-5) in the isolation of influenza A virus from nasopharyngeal aspirates by shell vial culture

Author:

Reina J1,Fernandez-Baca V1,Blanco I1,Munar M1

Affiliation:

1. Virology Unit, Clinical Microbiology Service, University Hospital Son Dureta (UIB), Palma de Mallorca, Spain.

Abstract

We report a comparative study of the MDCK, Vero, and MRC-5 cell lines in the isolation of the influenza A (IA) virus. We studied 746 samples in which 63 IA viruses were isolated. The MDCK line displayed 100% sensitivity, the Vero line displayed 71.4%, and the MRC-5 displayed 57.1%. The MDCK line showed a statistically significant difference with respect to the Vero line (P = 0.001) and the MRC-5 line (P = 0.001). The quantitative sensitivity analysis showed the MDCK line to be superior to the other lines. It seems that the MDCK line is still one of the most recommendable for the isolation of the IA virus from respiratory samples.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference13 articles.

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3. Isolation of influenza virus in human Iung embryonated fibroblast cells (MRC-5) from clinical samples;De Oa M.;J. Clin. Microbiol.,1995

4. Comparison of different tissue cultures for isolation and quantitation of influenza and parainfluenza viruses;Frank A. L.;J. Clin. Microbiol.,1979

5. Glezen W. P. and J. D. Cherry. 1992. Influenza viruses p. 1688-1704. In R. D. Feigin and J. D. Cherry (ed.) Textbook of pediatric infectious diseases 3rd ed. W. B. Saunders Co. Philadelphia Pa.

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