Siderophore-Mediated Iron Acquisition Influences Motility and Is Required for Full Virulence of the Xylem-Dwelling Bacterial Phytopathogen Pantoea stewartii subsp. stewartii

Abstract

ABSTRACTIron is a key micronutrient for microbial growth but is often present in low concentrations or in biologically unavailable forms. Many microorganisms overcome this challenge by producing siderophores, which are ferric-iron chelating compounds that enable the solubilization and acquisition of iron in a bioactive form.Pantoeastewartiisubsp.stewartii, the causal agent of Stewart's wilt of sweet corn, produces a siderophore under iron-limiting conditions. The proteins involved in the biosynthesis and export of this siderophore are encoded by theiucABCD-iutAoperon, which is homologous to the aerobactin biosynthetic gene cluster found in a number of enteric pathogens. Mutations iniucAandiutAresulted in a decrease in surface-based motility thatP. stewartiiutilizes during the early stages of biofilm formation, indicating that active iron acquisition impacts surface motility forP. stewartii. Furthermore, bacterial movementin plantais also dependent on a functional siderophore biosynthesis and uptake pathway. Most notably, siderophore-mediated iron acquisition is required for full virulence in the sweet corn host, indicating that active iron acquisition is essential for pathogenic fitness for this important xylem-dwelling bacterial pathogen.