Affiliation:
1. Plum Island Animal Disease Center, Science and Education Administration, U.S. Department of Agriculture, Greenport, New York 11944
Abstract
Chemical agents reported to inhibit the growth of various ribonucleic acid and deoxyribonucleic acid viruses were tested against foot-and-mouth disease virus in cell culture. These included Zn
2+
, aurintricarboxylic acid, polyribocytidylic acid, polyriboinosinic acid, phosphonoacetic acid, and the viral contact inactivator
N
-methyl isatin β-thiosemicarbazone alone and with CuSO
4
. The most effective agent, Zn
2+
, inhibited foot-and-mouth disease virus production in primary calf kidney cells by 1 log unit at 0.05 mM Zn
2+
and completely at 0.50 mM. Zinc was inhibitory even when added late in infection and was nontoxic to uninfected cells as measured by protein and nucleic acid syntheses. Polyacrylamide gel patterns of [
35
S]methionine-labeled, virus-specific proteins showed increasing amounts of higher-molecular-weight material, in accord with reports that Zn
2+
inhibits post-translational cleavages of other picornavirus precursor polypeptides.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Cited by
19 articles.
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