Rapid and Simultaneous Detection of bla KPC and bla NDM by Use of Multiplex Real-Time PCR

Abstract

ABSTRACT The increasing incidence of carbapenem nonsusceptibility among clinically important species is of global concern. Identification of the molecular mechanisms underlying carbapenem nonsusceptibility is critical for epidemiological investigations. In this report, we describe a real-time PCR-based assay capable of simultaneously detecting bla KPC and bla NDM , two of the most important carbapenemases, directly from culture in less than 90 min. The assay was validated with bla KPC - and bla NDM -carrying clinical isolates and demonstrated 100% concordance with the Carba NP test.