Differentiation of Neisseria gonorrhoeae isolates requiring proline, citrulline, and uracil by plasmid content, serotyping, and pulsed-field gel electrophoresis

Author:

Ng L K1,Carballo M1,Dillon J A1

Affiliation:

1. National Laboratory for Sexually Transmitted Diseases, Laboratory Centre for Disease Control, Health Canada, Ottawa, Ontario.

Abstract

A combination of DNA macrorestriction analysis using pulsed-field gel electrophoresis and a serotyping method using three panels of monoclonal antibody was used to discriminate 43 epidemiologically unrelated Neisseria gonorrhoeae isolates requiring proline, citrulline, and uracil (PCU-) into 35 groups. This indicates that PCU- isolates of N. gonorrhoeae are not clonal.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference17 articles.

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3. Physical map of the chromosome of Neisseria gonorrhoeae FA1090 with locations of genetic markers including opa and pil genes;Dempsey J. F.;J. Bacteriol.,1991

4. Serological ecology of Neisseria gonorrhoeae (PPNG and non-PPNG) strains: Canadian perspective;Dillon J. R.;Genitourin. Med.,1987

5. Auxotypes, plasmid contents, and serovars of gonococcal strains (PPNG and non-PPNG) from Jamaica;Dillon J. R.;Genitourin. Med.,1987

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