Affiliation:
1. Human Genome Center, Lawrence Berkeley Laboratory, Berkeley, California 94720, USA.
Abstract
Serial isolates of Candida albicans were obtained from 20 patients with leukemia over periods of up to 8 months. The fingerprinting of these isolates by interrepeat PCR and random amplified polymorphic DNA PCR has been described previously (A. van Belkum, W. Melchers, B. E. de Pauw, S. Scherer, W. Quint, and J. F. Meis, J. Infect. Dis. 169:1062-1070, 1994). Contour-clamped homogeneous electric field gel electrophoresis was used to examine the chromosomes of these isolates. When changes in the karyotype were seen in a series of isolates, additional interrepeat PCR and Southern blotting with a repeat DNA sequence from the 27A family were performed. These two genotyping tools were used to determine if karyotypic changes seen in a series of isolates were due to chromosome rearrangements in a single strain or due to colonization with more than one strain. It was determined that changes in karyotype in a series of strains indicated infection by a new strain.
Publisher
American Society for Microbiology
Reference14 articles.
1. Electrophoretic karyotypes of clinically isolated yeasts of Candida albicans and C. glabrata;Asakura K.;J. Gen. Microbiol.,1991
2. Candida albicans genotyping in studies with patients with AIDS developing resistance to fluconazole;Bart-Delabesse E.;J. Clin. Microbiol.,1993
3. Induced chromosome rearrangements and morphologic variation in Candida albicans;Barton R. C.;J. Bacteriol.,1994
4. Barton R. C. and S. Scherer. Unpublished data.
5. Barton R. C. D. Schmidt and S. Scherer. 1994. Analysis of karyotype in Candida albicans p. 99-104. In B. Maresca and G. S. Kobayashi (ed.) Molecular biology of pathogenic fungi: a laboratory manual. Telos Press New York.
Cited by
30 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献