Evaluation of lacto-phenol cotton blue for wet mount preparation of feces

Author:

Parija S C1,Prabhakar P K1

Affiliation:

1. Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, India.

Abstract

The use of lacto-phenol cotton blue (LPCB) stain for wet mount preparation of stools to demonstrate intestinal parasites by routine microscopy was evaluated in this study. LPCB-stained trophozoites and cysts and helminthic ova could easily be detected and identified in LPCB wet mounts of stools. The stain is recommended for routine use in the wet mount preparation of stools in a parasitology laboratory.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference8 articles.

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2. An easy and rapid method for detecting protozoal cysts in feces by means of wet stained preparations;Donaldson R.;Lancet,1917

3. Amebiasis: epidemiological studies in the United States, 1971-1974;Krogstad D. J.;Ann. Intern. Med.,1978

4. Melvin D. M. and M. M. Brooke. 1974. Laboratory procedures for the diagnosis of intestinal parasites. U.S. Department of Health Education and Welfare Publication no. (CDC) 75-8282. Center for Disease Control Washington D.C.

5. Rapid staining of intestinal amoebae on wet mounts;Nair C. P.;Nature London,1953

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