Affiliation:
1. Animal Research Center, Tokyo Medical College, Shinjuku, Japan. kawamoto@tokyo-med.ac.jp
Abstract
A suitable medium for the transport of Pasteurella multocida in nasal specimens from rabbits was investigated by using pure cultures of the organism and nasal swabs from infected rabbits. First, the ability of eight transport media to preserve the viabilities of P. multocida strains isolated from rabbits was studied. Cary-Blair medium and Leibovitz medium no. 15 (L-15) were found to be superior to the other six media tested, enabling survival of the organism for more than 14 days at room temperature. Second, the survival of P. multocida in nasal specimens was evaluated on both Cary-Blair medium and L-15. The recovery rate of the organism from these two media was more than 80 to 90% during 4 days of storage and decreased gradually with increasing preservation time. There were no significant differences (P > 0.05) in recovery rates of the organism between Cary-Blair medium and L-15. On the basis of these results, we recommend the use of Cary-Blair medium for the transport of P. multocida in rabbit nasal specimens because of the ease of transport of nasal swabs by mail.
Publisher
American Society for Microbiology
Reference20 articles.
1. Clear liquid mediums for the "aerobic;Brewer J. H.;JAMA,1940
2. New transport medium for shipment of clinical specimens. I. Fecal specimens;Cary S. G.;J. Bacteriol.,1964
3. Comparison of methods for the sampling and isolation of toxigenic Pasteurella multocida from the nasal cavity of pigs;Chanter N.;Res. Vet. Sci.,1989
4. Capsular and somatic types of Pasteurella multocida from rabbits;Chengappa M. M.;Can. J. Comp. Med.,1982
5. Cottral G. E. 1978. Collection and processing of specimens p. 35-46. In G. E. Cottral (ed.) Manual of standardized methods for veterinary microbiology. Cornell University Press Ithaca N.Y.
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