Affiliation:
1. Department of Biologics Research, Division of Communicable Disease and Immunology, Water Reed Army Institute of Research, Washington, D. C.
Abstract
Conklin, David
A. (Walter Reed Army Institute of Research, Washington, D. C.),
Marion E. Webster, Patricia L. Altieri, Sanford Berman, Joseph P. Lowenthal, and Raymond B. Gochenour
. Large scale purification of proteinases from
Clostridium histolyticum
filtrates. J. Bacteriol.
82:
589–594. 1961.—A method for the large scale preparation and partial purification of
Clostridium histolyticum
proteinases by fractional precipitation with ammonium sulfate is described. Conditions for adequate separation and purification of the δ-proteinase and the gelatinase were obtained. Collagenase, on the other hand, was found distributed in four to five fractions and little increase in purity was achieved as compared to the crude ammonium sulfate precipitates.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
6 articles.
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