Dissimilatory Selenate Reduction Potentials in a Diversity of Sediment Types

Abstract

We measured potential rates of bacterial dissimilatory reduction of 75 SeO 4 2− to 75 Se 0 in a diversity of sediment types, with salinities ranging from freshwater (salinity = 1 g/liter) to hypersaline (salinity = 320 g/liter and with pH values ranging from 7.1 to 9.8. Significant biological selenate reduction occurred in all samples with salinities from 1 to 250 g/liter but not in samples with a salinity of 320 g/liter. Potential selenate reduction rates (25 nmol of SeO 4 2− per ml of sediment added with isotope) ranged from 0.07 to 22 μmol of SeO 4 2− reduced liter −1 h −1 . Activity followed Michaelis-Menten kinetics in relation to SeO 4 2− concentration ( K m of selenate = 7.9 to 720 μM). There was no linear correlation between potential rates of SeO 4 2− reduction and salinity, pH, concentrations of total Se, porosity, or organic carbon in the sediments. However, potential selenate reduction was correlated with apparent K m for selenate and with potential rates of denitrification ( r = 0.92 and 0.81, respectively). NO 3 − , NO 2 − , MoO 4 2− , and WO 4 2− inhibited selenate reduction activity to different extents in sediments from both Hunter Drain and Massie Slough, Nev. Sulfate partially inhibited activity in sediment from freshwater (salinity = 1 g/liter) Massie Slough samples but not from the saline (salinity = 60 g/liter) Hunter Drain samples. We conclude that dissimilatory selenate reduction in sediments is widespread in nature. In addition, in situ selenate reduction is a first-order reaction, because the ambient concentrations of selenium oxyanions in the sediments were orders of magnitude less than their K m s.