Affiliation:
1. Lehrstuhl für Mikrobiologie, Universität Bayreuth, D-8580 Bayreuth, and Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, D-3300 Braunschweig 2, 2 Federal Republic of Germany
Abstract
The novel thermophilic CO- and H
2
-oxidizing bacterium UBT1 has been isolated from the covering soil of a burning charcoal pile. The isolate is gram positive and obligately chemolithoautotrophic and has been named
Streptomyces thermoautotrophicus
on the basis of G+C content (70.6 ± 0.19 mol%), a phospholipid pattern of type II, MK-9(H
4
) as the major quinone, and other chemotaxonomic and morphological properties.
S. thermoautotrophicus
could grow with CO (
t
d
= 8 h), H
2
plus CO
2
(
t
d
= 6 h), car exhaust, or gas produced by the incomplete combustion of wood. Complex media or heterotrophic substrates such as sugars, organic acids, amino acids, and alcohols did not support growth. Molybdenum was required for CO-autotrophic growth. For growth with H
2
, nickel was not necessary. The optimum growth temperature was 65°C; no growth was observed below 40°C. However, CO-grown cells were able to oxidize CO at temperatures of 10 to 70°C. Temperature profiles of burning charcoal piles revealed that, up to a depth of about 10 to 25 cm, the entire covering soil provides a suitable habitat for
S. thermoautotrophicus
. The
K
m
was 88 μl of CO liter
−1
and
V
max
was 20.2 μl of CO h
−1
mg of protein
−1
. The threshold value of
S. thermoautotrophicus
of 0.2 μl of CO liter
−1
was similar to those of various soils. The specific CO-oxidizing activity in extracts with phenazinemethosulfate plus 2,6-dichlorophenolindophenol as electron acceptors was 246 μmol min
−1
mg of protein
−1
. In exception to other carboxydotrophic bacteria,
S. thermoautotrophicus
CO dehydrogenase was able to reduce low potential electron acceptors such as methyl and benzyl viologens.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference50 articles.
1. Techniques for the preservation of threedimensional structure in preparing specimens for the electron microscope;Anderson T. F.;Trans. N. Y. Acad. Sci.,1951
2. Bell J. M. E. Williams and J. Colby. 1985. Carbon monoxide oxidoreductases from thermophilic carboxydobacteria p. 153-160. In C. K. Poole and C. S. Dow (ed.) Microbial gas metabolism. Academic Press Inc. (London) Ltd. London.
3. A rapid alkaline extraction procedure for screening recombinant plasmid DNA;Birnboim H. C.;Nucleic Acids Res.,1979
4. Brocksiepe H. G. 1976. Holzverkohlung p. 703-708. In E. Bartholome E. Biekert H. Hellmann H. Ley and W. M. Weigert (ed.) Ullmanns Enzyklopadie der technischen Chemie vol. 12. Verlag Chemie New York.
5. Role of carboxydobacteria in consumption of atmospheric carbon monoxide by soil;Conrad R.;Appl. Environ. Microbiol.,1981
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