INDUCTION AND REPRESSION OF l -ARABINOSE ISOMERASE IN PEDIOCOCCUS PENTOSACEUS

Abstract

Dobrogosz, Walter J. (University of Illinois, Urbana) and Ralph D. DeMoss . Induction and repression of l -arabinose isomerase in Pediococcus pentosaceus . J. Bacteriol. 85: 1350–1355. 1963.—The inducible l -arabinose isomerase of Pediococcus pentosaceus can be rapidly and conveniently measured in whole-cell preparations by use of a standard colorimetric procedure originally developed for studies with cell-free enzyme preparations. The enzyme is measured by its ability to catalyze the isomerization of l -arabinose to l -ribulose. Whole cells suspended in a suitable buffer and pretreated with toluene were shown to exhibit this isomerase activity at a level comparable with that observed in cell-free enzyme preparations. Conditions for optimal induction of l -arabinose isomerase are described. In addition, it was determined that the formation of this enzyme is subject to repression by glucose, i.e., via catabolite repression.