Affiliation:
1. Department of Biochemistry, University of Otago, Dunedin, New Zealand.
Abstract
The gene (PRA11) encoding a secreted aspartate proteinase of Candida albicans has been cloned and sequenced. The nucleotide and deduced amino acid sequences of PRA11 are 77 and 73% identical, respectively, with the reported sequences of PRA10 also cloned from C. albicans. Southern analyses indicated that the genome of each strain examined (ATCC 10231 and ATCC 10261) contains PRA10 and PRA11. Northern (RNA) analyses showed that PRA11 was expressed at a much higher level than was PRA10 when secretion of the proteinase by strain ATCC 10261 was induced with albumin.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference34 articles.
1. PEP4 gene of Saccharomyces cerevisiae encodes proteinase A, a vacuolar enzyme required for processing of vacuolar precursors;Ammerer G.;Mol. Cell. Biol.,1986
2. Ausubel F. M. R. Brent R. E. Kingston D. D. Moore J. A. Smith J. G. Seidman and K. Strubl. 1987. Current protocols in molecular biology. John Wiley & Sons New York.
3. Molecular mechanisms of protein secretion and the role of the signal sequence;Briggs M. S.;Adv. Protein Sequencing,1986
4. Brown T. A. 1991. Molecular biology LabFax. BIOS Scientific Publishers Oxford.
5. Proteases and the processing of precursors to secreted proteins in yeast;Bussey H.;Yeast,1988
Cited by
79 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献