Affiliation:
1. Institute of Molecular Genetics and Genetic Engineering, Belgrade, Yugoslavia.
Abstract
The sisomicin-gentamicin resistance methylase (sgm) gene was isolated from Micromonospora zionensis and cloned in Streptomyces lividans. The sgm gene was expressed in Micromonospora melanosporea, where its own promoter was active, and also in Escherichia coli under the control of the lacZ promoter. The complete nucleotide sequence of 1,122 bp and a transcription start point were determined. The sequence contains an open reading frame that encodes a polypeptide of 274 amino acids. The methylation of 30S ribosomal subunits by Sgm methylase accounts adequately for all known resistance characteristics of M. zionensis, but expression of high-level resistance to hygromycin B is background dependent. A comparison of the amino acid sequence of the predicted Sgm protein with the deduced amino acid sequences for the 16S rRNA methylases showed extensive similarity of Grm and significant similarity to KgmB but not to KamB methylase.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference32 articles.
1. Temporally regulated tandem promoters in Micromonospora echinospora;Baum E. Z.;J. Bacteriol.,1988
2. Site of action of two ribosomal RNA methylases responsible for resistance to aminoglycosides;Beauclerk A. A. D.;J. Mol. Biol.,1987
3. Berdy J. and M. Jarai. 1986. Micromonospora produced aminoglycoside antibiotics: chemistry and microbiology. Process Biochem. June 93-100.
4. How antibiotic-producing organisms avoid suicide. Annu;Cundliffe E.;Rev. Microbiol.,1989
5. Cundliffe E. 1990. Recognition sites for antibiotics within rRNA p. 479-490. In W. E. Hill A. Dahlberg R. A. Garrett P. B. Moore D. Schlessinger and J. R. Warner (ed.) The ribosome: structure function and evolution. American Society for Microbiology Washington D.C.
Cited by
33 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献