Conversion of Dexon (
p
-Dimethylaminobenzenediazo Sodium Sulfonate) to
N,N
-Dimethyl-
p
-Phenylenediamine by
Pseudomonas fragi
Bk9
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Published:1974-01
Issue:1
Volume:27
Page:43-46
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ISSN:0003-6919
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Container-title:Applied Microbiology
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language:en
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Short-container-title:Appl Microbiol
Author:
Karanth N. G. K.12,
Bhat S. G.12,
Vaidyanathan C. S.12,
Vasantharajan V. N.12
Affiliation:
1. Microbiology and Pharmacology Laboratory, Indian Institute of Science, Bangalore 560012, India
2. Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India
Abstract
The metabolism of the fungicide Dexon (
p
-dimethylaminobenzenediazo sodium sulfonate) by a soil bacterium is reported for the first time. The organism which is capable of using Dexon only by a co-metabolic process was obtained by enrichment culture and was identified as
Pseudomonas fragi
. The first metabolic product of Dexon was identified as
N,N
-dimethyl-
p
-phenylenediamine. The presence of an enzyme,
p
-dimethylaminobenzenediazo sodium sulfonate reductase, capable of reducing Dexon to
N,N
-dimethyl-
p
-phenylenediamine has been demonstrated in the cell-free extracts of the organism. The enzyme is found to be in the soluble fraction and requires dithiothreitol as a reductant.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
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