Solid-Phase Radioimmunoassay for Detecting Bovine (Neonatal Calf Diarrhea) Rotavirus Antibody-Reference-Cited by-同舟云学术

Solid-Phase Radioimmunoassay for Detecting Bovine (Neonatal Calf Diarrhea) Rotavirus Antibody

Published:1977-07 Issue:1 Volume:6 Page:10-15
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Babiuk Lorne A.¹,Acres Stephen D.,Rouse Barry T.

Affiliation:

1. Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, Saskatchewan, S7N 0W0, Canada

Abstract

An indirect solid-phase microradioimmunoassay is described for detecting antibodies against rotaviruses. The test involved ethanol fixation of microcultures of bovine rotavirus-infected BSC-1 cells and reaction with bovine antirotavirus serum, followed by 125 I-labeled rabbit anti-bovine immunoglobulin G. The technique was shown to be virus specific and highly sensitive. The fixed microcultures could be stored at 4°C for at least 2 months without affecting the sensitivity of the test. The application of this system for the detection of rotavirus antibodies in humans is briefly discussed.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.6.1.10-15.1977

Reference22 articles.

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