Detection of "neutral" type 7F and type 14 pneumococcal capsular polysaccharides by immunoelectrophoresis

Author:

Szu S C,Oravec L S

Abstract

Type 7F and type 14 pneumococcal capsular polysaccharides, neutral at pH 8.6, were studied by immunoelectrophoresis at pH 5. Three techniques were used: rocket, countercurrent, and reversed immunoelectrophoresis. Our results show that these two capsular polysaccharides types can be detected at pH 5 with high sensitivity.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference12 articles.

1. Counterimmunoelectrophoresis of pneumococcal antigens: improved sensitivity for the detection of types VII and XIV;Anhalt J. P.;J. Clin. Microbiol.,1975

2. Quantitation of human IgG by rocket immunoelectrophoresis at pH 5 by use of carbamylated antibodies. A routine laboratory method;Bjerrum 0.;Clin. Chim. Acta,1973

3. Immunoelectrophoresis for detection of polysaccharides in immune complexes;Coonrod J. D.;J. Clin. Microbiol.,1978

4. Detection of typespecific pneumococcal antigens by counterimmunoelectrophoresis. I. Methodology and immunologic properties of pneumococcal antigens;Coonrod J. D.;J. Lab. Clin. Med.,1973

5. Coonrod J. D. and M. W. Rytel. 1973. Detection of type-NOTES 1175

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