Affiliation:
1. Physical Defense Division, U.S. Army Biological Laboratories, Fort Detrick, Frederick, Maryland
Abstract
Huston, Charles
K. (Fort Detrick, Frederick, Md.),
Phillip W. Albro, and Gerald B. Grindey
. Lipids of
Sarcina lutea
. III. Composition of the complex lipids. J. Bacteriol.
89:
768–775. 1965.—The complex lipids from a strain of
Sarcina lutea
were isolated and separated into fractions on diethylaminoethyl cellulose acetate and silicic acid columns. These fractions were monitored in several thin-layer chromatography systems. The various lipid types were characterized by their behavior in thin-layer systems and by an analysis of their hydrolysis products. The fatty acid composition of the column fractions was determined by gas-liquid chromatography. A number of components (13) were separated by thin-layer chromatography and characterized. The major components were polyglycerol phosphatide (17.0%), lipoamino acids (15.1%), phosphatidyl glycerol (13.8%), and an incompletely characterized substance (15.0%). Minor constituents included phosphatidyl inositol (5.5%), phosphatidic acid (4.2%), phosphatidyl serine (2.0%), and phosphatidyl choline (1.0%). No phosphatidyl ethanolamine was observed.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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