Affiliation:
1. Department of Bacteriology and Immunology, Harvard Medical School, Boston, Massachusetts
Abstract
Low, Iolanda
E. (Harvard Medical School, Boston, Mass.),
and Monroe D. Eaton
. Replication of
Mycoplasma pneumoniae
in broth culture. J. Bacteriol.
89:
725–728. 1965.—Reproducible growth curves of
Mycoplasma pneumoniae
can be obtained with the use of shaking cultures, which allows earlier and higher yields of the organism than stationary or roller cultures. Decreasing oxygen tension clearly decreases the growth of
M. pneumoniae
to barely detectable levels, with or without glucose or pyruvate as substrates. The use of phenol red as a
p
H indicator in the medium is helpful in judging the replication of a culture so that log-phase organisms can be harvested in large amounts.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference13 articles.
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2. CHANOCK R. M. W. D. JAMES H. H. Fox H. C.
3. Growth of Eaton PPLO in broth and preparation of complement-fixing antigen;LOW AN RNER;Proc. Soc. Exp. Biol. Med.,1962
4. Hemolysis in identifying Eaton's pleuropneumonia-like organism;CLYDE W. A.;Science,1963
5. CLYDE W. A. JR. 1964. Studies on the nature and properties of Eaton's agent (Mycoplasma pneumoniae). Bacteriol. Proc. p. 54.
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