Author:
Shields P A,Berenfeld S A,Farrah S R
Abstract
Enteroviruses added to 114 liters of dechlorinated tap water were recovered in a 16-ml sample by a two-stage concentration procedure in which different types of membrane filters were used in each concentration stage. Viruses in tap water at pH 3.5 were first adsorbed to 10-in. (ca. 25.4-cm) epoxy-fiber glass filters (Filterite). Viruses adsorbed to these filters were eluted with a solution of 0.2 M sodium trichloroacetate buffered at pH 9 with 0.2 M lysine. Viruses in this solution were adsorbed to 47-mm asbestos filters (Seitz) without pH adjustment or other modification of the solution. Viruses were recovered from the Seitz filters with 16 ml of either Casitone or fetal calf serum at pH 9. With these procedures ca. 45% of several types of enteroviruses added to 114 liters of tap water could be recovered in the final 16-ml sample.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference15 articles.
1. Farrah S. R. and G. Bitton. 1982. Methods (other than microporous filters) for concentration of viruses from water p. 117-150. In C. P. Gerba and S. M. Goyal (ed.) Methods in environmental virology. Marcel Dekker Inc. New York.
2. Concentration of viruses from large volumes of tap water using pleated membrane filters;Farrah S. R.;Appl. Environ. Microbiol.,1976
3. Effects of chaotropic and antichaotropic agents on elution of poliovirus adsorbed on membrane filters;Farrah S. R.;Proc. Natl. Acad. Sci. U.S.A.,1981
4. Goyal S. M. and C. P. Gerba. 1982. Concentration of viruses from water by membrane filters p. 59-116. In C. P. Gerba and S. M. Goyal (ed.) Methods in environmental virology. Marcel Dekker Inc. New York.
5. Organic flocculation: an efficient second-step concentration method for the detection of viruses in tap water;Katzenelson E.;Appl. Environ. Microbiol.,1976
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