Cell Cycle-Dependent Switch of TopBP1 Functions by Cdk2 and Akt-Reference-Cited by-同舟云学术

Cell Cycle-Dependent Switch of TopBP1 Functions by Cdk2 and Akt

Published:2020-03-30 Issue:8 Volume:40 Page:
ISSN:0270-7306
Container-title:Molecular and Cellular Biology
language:en
Short-container-title:Mol Cell Biol

Author:

Liu Kang¹,Graves Joshua D.¹²,Lee Yu-Ju¹³,Lin Fang-Tsyr¹,Lin Weei-Chin¹²³⁴

Affiliation:

1. Section of Hematology/Oncology, Department of Medicine, Baylor College of Medicine, Houston, Texas, USA

2. Integrative Molecular and Biomedical Sciences Graduate Program, Baylor College of Medicine, Houston, Texas, USA

3. Interdepartmental Program in Translational Biology and Molecular Medicine, Baylor College of Medicine, Houston, Texas, USA

4. Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, USA

Abstract

Cdk2-dependent TopBP1-treslin interaction is critical for DNA replication initiation. However, it remains unclear how this association is terminated after replication initiation is finished. Here, we demonstrate that phosphorylation of TopBP1 by Akt coincides with cyclin A activation during S and G 2 phases and switches the TopBP1-interacting partner from treslin to E2F1, which results in the termination of replication initiation. Premature activation of Akt in G 1 phase causes an early switch and inhibits DNA replication.

Funder

HHS | National Institutes of Health

U.S. Department of Defense

Publisher

American Society for Microbiology

Subject

Cell Biology,Molecular Biology

Link

https://journals.asm.org/doi/pdf/10.1128/MCB.00599-19

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