Agents That Inhibit Rho, Rac, and Cdc42 Do Not Block Formation of Actin Pedestals in HeLa Cells Infected with Enteropathogenic Escherichia coli-Reference-Cited by-同舟云学术

Agents That Inhibit Rho, Rac, and Cdc42 Do Not Block Formation of Actin Pedestals in HeLa Cells Infected with Enteropathogenic Escherichia coli

Published:1998-04 Issue:4 Volume:66 Page:1755-1758
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Ben-Ami Goni¹,Ozeri Vered²,Hanski Emanuel²,Hofmann Fred³,Aktories Klaus³,Hahn Klaus M.⁴,Bokoch Gary M.⁴⁵,Rosenshine Ilan¹

Affiliation:

1. Departments of Molecular Genetics and Biotechnology1 and of

2. Clinical Microbiology,2 Faculty of Medicine, The Hebrew University, Jerusalem 91120, Israel;

3. Institute of Pharmacology and Toxicology, Albert-Ludwigs University, Freiburg, Germany3; and

4. Departments of Cell Biology4 and of

5. Immunology,5 The Scripps Research Institute, La Jolla, California 92037

Abstract

ABSTRACT Enteropathogenic Escherichia coli (EPEC) induces formation of actin pedestals in infected host cells. Agents that inhibit the activity of Rho, Rac, and Cdc42, including Clostridium difficile toxin B (ToxB), compactin, and dominant negative Rho, Rac, and Cdc42, did not inhibit formation of actin pedestals. In contrast, treatment of HeLa cells with ToxB inhibited EPEC invasion. Thus, Rho, Rac, and Cdc42 are not required for assembly of actin pedestals; however, they may be involved in EPEC uptake by HeLa cells.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.66.4.1755-1758.1998

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