Affiliation:
1. Department of Biochemistry and Molecular Genetics, University of Colorado Health Science Center at Fitzsimons, Aurora, Colorado
Abstract
ABSTRACT
We investigated the role of RNA polymerase II (pol II) carboxy-terminal domain (CTD) phosphorylation in pre-mRNA processing coupled and uncoupled from transcription in
Xenopus
oocytes. Inhibition of CTD phosphorylation by the kinase inhibitors 5,6-dichloro-1β-
d
-ribofuranosyl-benzimidazole and H8 blocked transcription-coupled splicing and poly(A) site cleavage. These experiments suggest that pol II CTD phosphorylation is required for efficient pre-mRNA splicing and 3′-end formation in vivo. In contrast, processing of injected pre-mRNA was unaffected by either kinase inhibitors or α-amanitin-induced depletion of pol II. pol II therefore does not appear to participate directly in posttranscriptional processing, at least in frog oocytes. Together these experiments show that the influence of the phosphorylated CTD on pre-mRNA splicing and 3′-end processing is mediated by transcriptional coupling.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
Cited by
103 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献